HPLC Method Development, Tips & Troubleshooting
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Access practical guidance for HPLC and LC-MS method development, troubleshooting, column care, mobile phase optimization, and chromatographic performance improvement. Explore real-world solutions, best practices, and technical insights to improve analytical results and laboratory efficiency.
Method development guidance
LC-MS optimization
Mobile phase selection
Column care and recovery
Peak shape troubleshooting
Retention troubleshooting
HILIC and ANP strategy
Sample preparation advice
Instrument performance guidance
Real-world chromatography problem solving
Acid Concentration Optimization in HILIC Methods Using Cogent TYPE-C Columns - Tech Information
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Activity of a Stationary Phase in Chromatography Definition and Impact on HPLC Separations - Tech Information
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Amide vs Amino HPLC Columns Differences in Stability Selectivity and Performance - Tech Information
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Ammonium Acetate and Ammonium Formate in Acetonitrile Rich HILIC Mobile Phases - Tech Information
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Ammonium Acetate in LC-MS Methods Preventing Source Contamination and Signal Loss - Tech Information
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Avoid Distorted Peak Shapes When Using a Basic Mobile Phase pH for the Analysis of a Lipophilic Amine - Tips & Suggestions
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Avoid Ion Suppression in LC-MS When Ion-Pair Reagents Are Traditionally Required - HPLC Primer
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Avoid Spurious Peaks from Nylon Syringe Filters When Using Electrochemical Detection - Tech Information
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Baseline Stability in HILIC Methods Using Cogent Diamond Hydride Columns - Tech Information
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Broad and Asymmetrical Peak Troubleshooting in Reversed-Phase LC-MS Methods - Tech Information
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Bubble Point Pressure and Filtering Water Through Hydrophobic Membranes - Tech Information
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Buffer Concentration Effects on Acid Retention in HILIC Methods Using TYPE-C™ Columns - Tech Information
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Cocaine and Polar Metabolites ( Ecgonine) Separation using LC‑MS / MS - Tips and Suggestions
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Column Particle Damage as a Cause of Frit Blockage and Elevated HPLC Backpressure - Tech Information
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Column Void Volume and Capacity Factor Calculations for HPLC Method Transfer - Tech Information
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Constant Pressure Method on two Instruments Producing Different Flow Rates- Troubleshooting
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Converting 0.1% w/v Ammonium Acetate to Millimolar Concentration for HPLC and LC-MS - Tech Information
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Crimped Cap Removal from Fused Insert Vials Without Damaging the Insert - Tech Information
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Effects of Flow Rate on Peak Area Response Decline in HPLC UV Detection - Tech Information
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Effects of Mobile Phase Ionic Strength in HILIC Methods Using Cogent TYPE-C Columns - Tech Information
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Effects of Washing and Reusing Autosampler Vials on Sample Recovery and Contamination Risk - Tech Information
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Eliminating Retention Time Drift in LC-MS HILIC Methods Using Diamond Hydride Columns - Tech Information
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Formic Acid Improves Peak Shapes Retention and Carryover for Polar Compounds with the Cogent Diamond Hydride HPLC Column - Tips & Suggestions
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Gradient Method Adjustments When Changing HPLC Column Internal Diameter - Tech Information
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High HPLC System Pressure Originating from the Pump Module Troubleshooting - Tech Information
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High Pressure Develops after Flushing a Cogent Diamond Hydride HPLC Column with Acetonitrile Cause and Solution - Tech Information
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HILIC Methods Using Cogent TYPE-C Columns Compared to Conventional HILIC Columns - Tech Information
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Holmium Oxide and Caffeine Standards for UV-Vis and HPLC Detector Qualification Below 230 nm - Tech Information
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How Tight Should an Autosampler Vial Cap Be to Prevent Septa Push-Through During Injection - Tech Information
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How to keep HPLC columns free of adsorbed Preventing Contaminant Buildup and Maintaining HPLC Column Performance - Tech Informationcontaminants - How To
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HPLC and LC-MS Method Validation Fundamentals for Regulated Laboratories - Tech Information
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Improving Accuracy and Precision for Polar Compound Analysis Using HILIC Methods and TYPE-C Columns - Tech Information
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Improving Citric Acid Retention and Peak Shape Reproducibility in LC-MS Analysis - Tech Information
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Improving LC-MS Sensitivity with HILIC Methods for Polar Compound Analysis - Tech Information
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Improving Metformin Reproducibility and Peak Shape in Plasma Sample Analysis - Tech Information
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Improving Peak Shape and Retention for Organic Acids in HILIC Methods Using Cogent Diamond Hydride Columns - Tech Information
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Improving Peak Shape for Phosphorylated Compounds in HILIC Methods Using TYPE-C Columns - Tech Information
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Improving Polar Compound Analysis with TYPE-C HILIC Columns for LC and LC-MS - Tech Information
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Improving Retention and Peak Shape Reproducibility for Citric Acid Analysis - Tech Information
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Increasing Selectivity and Resolution for a Xylose and Ribose HPLC Method Resolution - Tips and Suggestions
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Intermittent Pressure Spikes in HPLC Systems and How to Troubleshoot Them - Tech Information
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L3 Linearity Solution to Assess UV-Visible Detector Wavelength Accuracy - Tech Information
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Lisinopril Assay Results Calculation Using a Dihydrate Reference Standard - Tech Information
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Lower Retention When Functional Group in a Compound Should be Ionized - Troubleshooting Tips
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Managing PFAS and Trace Contamination Sources in Chromatographic Analysis - Tech Information
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Maximum Operating Pressure Ratings for Cogent HPLC Columns by Particle Size - Tech Information
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Maximum Salt Concentration Recommendations for Cogent TYPE-C HPLC Columns - Tech Information
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MRQ and Max Recovery Autosampler Vials Functional Differences and Selection Considerations - Tech Information
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No Peaks Observed for Succinic Acid or Alpha-Ketoglutarate in LC-MS Using a Cogent Diamond Hydride Column - Tech Information
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PFAS Analysis Selecting Glass or Polypropylene Vials for Sampling, Storage and LC-MS Analysis - Tech Information
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Positive and Negative Ion Mode Mobile Phase Additives for LC-MS Method Development - Tech Information
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Preparing 10 mM Ammonium Acetate Mobile Phases in High-Acetonitrile HILIC Methods - Tech Information
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Preparing a 16 mM Ammonium Acetate Mobile Phase in 90 10 Acetonitrile Water for HPLC and LC-MS - How To
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Preparing Premixed Diluents to Improve Accuracy in HPLC Sample Preparation - Tech Information
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Restoring the Initial Method Results Analyzing AMP ADP ATP UDP and GPT in Biological Extracts - Troubleshooting
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Retaining Polar Compounds with Cogent Bidentate C18 Columns Reversed Phase and HILIC Considerations - Tech Information
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Retention of Ionized Polar Compounds in HILIC Methods Using Cogent TYPE-C Columns - Tech Information
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Retention Time Variability Caused by Mobile Phase Mixing Inconsistencies in HPLC Systems - Tech Information
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Retention Times Changes with Mobile Phase Storage Effects on Retention Time and Peak Shape in HPLC Methods - Tech Information
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Ribose and Xylose Analysis Using Amide HILIC Columns Without Schiff Base Formation - AppNote
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Sample Loading Capacity and Injection Volume Guidelines for Semi-Preparative Cogent TYPE-C HPLC Columns - Tech Information
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Selecting Organic Solvents for HILIC Methods Using Cogent TYPE-C Columns - Tech Information
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Selectivity Modification in an HILIC Method Using Different Mobile Phase pH - Tips & Suggestions
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Separating Hydrophilic and Hydrophobic Compounds in the Same HILIC Method - Tech Information
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Sodium and Ammonium Adduct Formation for Improved Sugar Detection in LC-MS - Tech Information
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Sodium Ions and Sodium Succinate Analysis in HILIC Methods Using Cogent TYPE-C Columns - Tech Information
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Starting Flow Rate Guidelines for Common HPLC Column Internal Diameters - Tech Information
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Storage Conditioning and Solvent Transition Guidelines for Cogent TYPE-C HPLC Columns - Tech Information
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Storing, Conditioning, and Maintaining Cogent Diamond Hydride HPLC Columns - Tech Information
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Switching Between Reversed Phase and Normal Phase Methods on Cogent TYPE C Silica Columns - Tech Information
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System Pressure Considerations When Using 90% Methanol and 10% Water with Cogent TYPE-C HPLC Columns - Tech Information
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Tandem HPLC Columns in One Separation for Polar and Non Polar Compounds - Tips and Suggestions
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Tartaric and Malic Acid Separation in Complex Organic Acid Mixtures Using HILIC LC-MS- AppNote
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Temperature Operating Range of MICROSOLV Polypropylene Autosampler Screw Caps - Tech Information
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Troubleshooting Diminished Peak Height in Biological Extract Analysis Using Cogent Diamond Hydride Columns - Tech Information
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Understanding Polar Compound Retention Why LFER Models Often Provide More Insight Than Log D - Tech Information
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Understanding Retention Decreases as Water Content Increases on Cogent Bidentate C18 Columns - Tech Information
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Understanding the Impact of Extra Column Volume on HPLC Method Performance - Tech Information
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Using EDTA in Mobile Phases to Improve Peak Shape for Metal-Sensitive Compounds - Tech Information
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Using Isopropanol IPA in HILIC Mobile Phases with Cogent TYPE-C Columns - Tech Information
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